Mozzies are evolving to beat insecticides – except in Australia

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Mosquitoes are the main vectors for dengue and zika. Insecticides are our best weapon against them.
Anja Jonsson/Flickr

Ary Hoffmann, University of Melbourne; Nancy Margaret Endersby-Harshman, University of Melbourne, and Scott Ritchie, James Cook University

Chemical pesticides have been used for many years to control insect populations and remain the most important method of managing diseases carried by pests, including mosquitoes. However, insects have fought back by evolving resistance to many pesticides. There are now thousands of instances of evolved resistance, which make some chemical classes completely ineffective.

The Aedes mosquito, largely responsible for the spread of viruses like dengue and zika, has globally developed resistance to commonly used chemicals, including pyrethroids. Pyrethroids are the most used insecticides in the world, which includes the control of dengue outbreaks and quarantine breaches at air and sea ports.

In Asia and the Americas, pyrethroid resistance in Aedes mosquitoes is now widespread. In Australia, our mosquitoes have not developed these defences and pyrethroids are still very effective.

The difference lies in our stringent and careful protocols for chemical use. As the global community fights zika and other mosquito-borne diseases, there are lessons to be learned from Australia’s success.

Developing resistance

Mosquitoes usually become resistant to pyrethroids through the mutation of a sodium channel gene that controls the movement of ions across cell membranes. Mutations in a single gene are enough to make mosquitoes almost completely resistant to the level of pyrethroids used in insecticides.

The mutations first arises in a population by chance, and are rare. However, they rapidly spread as resistant females breed. The more times a mosquito population is exposed to the same chemical, the more the natural selection process favours their impervious offspring.

Eventually, when many individuals in a population carry the resistance mutation, the chemical becomes ineffective. This can happen where insecticide “fogging” is common practice. Overseas, fogging is sometimes undertaken across entire neighbourhoods, several times a month, despite concerns about its effectiveness as well as its environmental and health impacts.

A pest exterminator carries out insecticide fogging in an apartment block in Singapore.
EPA, Wallace Woon/AAP

Once resistance develops, it can spread to non-resistant mosquito populations in other areas. Pest species, including mosquitoes, are often highly mobile because they fly or are carried passively (in vehicles, ships and planes) at any stage of their life cycle. Their mobility means mutations spread quickly, crossing borders and possibly seas.

We can still control Australian mosquitoes

Despite this, Australian populations of Aedes mosquitoes remain susceptible to pyrethroids. Aedes aegypti (the yellow fever mosquito) is the main disease-carrying mosquito in Australia. Its population is restricted to urban areas of northern Queensland, where dengue can occur.

Recent research found that all Australian populations of this species are still vulnerable to pyrethroids. None of the hundreds of mosquitoes tested had any mutations in the sodium channel gene, despite the high incidence of such mutations in mosquito populations of South-East Asia.

A female Aedes aegypti mosquito during a feed.
James Gathany, CDC Prof Frank Hadley Collins/Wikimedia

We believe these mosquitoes remain vulnerable to pyrethroids because in Australia pressure to select for resistance has been low.

Australia does not carry out routine fogging. If dengue is detected in an area, pyrethoids are used in highly regimented and limited fashion. Spraying is restricted to the insides of premises within selected house blocks, and then only for a short period.

Importantly, water-filled artificial containers, which can serve as a habitat for larvae, are treated with insect growth regulators, which do not select for the pyrethroid resistance mutations.

Exporting resistance

With chemical resistance growing around the world, it is more urgent than ever that we co-ordinate action to control and reduce risk of resistance. Unfortunately, no global guidelines exist to minimise the evolution of resistance in mosquitoes.

Adopting pesticide resistance management strategies has proven to be effective against other pests – for example, the corn earworm (Helicoverpa armigera). Guidelines include rotating different class of pesticides to deny pests the chance to develop resistance, and investing in non-chemical options such as natural predators of target pests.

Resistance management strategies are particularly critical for new pesticides that have different modes of attack, such as preventing juvenile insects from moulting, or attacking various chemical receptors.

The ConversationTo prolong the effectiveness of pesticides, we must develop these strategies before resistance begins to develop. North Queensland may be an example to the rest of the world on the best path forward.

Ary Hoffmann, Professor, School of BioSciences and Bio21 Institute, University of Melbourne; Nancy Margaret Endersby-Harshman, Research fellow, University of Melbourne, and Scott Ritchie, Professorial Research Fellow, James Cook University

This article was originally published on The Conversation. Read the original article.

A tale of three mosquitoes: how a warming world could spread disease

David Yeates, CSIRO

Changing wildlife: this article is part of a series looking at how key species such as bees, insects and fish respond to environmental change, and what this means for the rest of the planet.

As the world warms, animals and plants will shift their ranges to keep pace with their favoured climate. While the changing distributions of species can tell us how climate change is affecting the natural world, it may also have a direct impact on us.

One good example is the disease carried by insects.

Those small, familiar flies called mosquitoes are responsible for much human suffering around the globe because of their ability to transmit diseases.

Mosquitoes transmit a number of viruses, such as Dengue, Ross River Virus, Murray Valley Encephalitis Virus and the local variant of the West Nile Virus known as Kunjin.

Could climate change cause these diseases to spread? While this an extremely important health question, the answer is far from simple.

Complicated life cycle

The life cycle of mosquitoes and its viral parasites is particularly complicated.

Only adult females consume blood, and the immature stages (larvae) live in fresh or brackish water, filtering out small organic particles.

The virus undergoes certain parts of its lifecycle inside particular mosquito organs, but also requires other organs in the vertebrate host to complete its life cycle. And to get into a vertebrate, such as us, it relies on a hungry blood-sucking insect.

These viruses always have other hosts besides humans, which may include native and domestic animals. The pathway that these viruses take to infect humans is often via our domestic animals, which are also bitten by the same mosquitoes that feed on us.

In addition, rates of virus transmission to humans is also affected by the human built environment, and also human behaviour.

Because mosquitoes breed in water, changes in rainfall patterns are likely to change the distribution and abundance of mosquitoes, and therefore could affect disease transmission.

The larvae of Aedes notoscriptus, which carries the Barmah Forest and Ross River viruses.
Stephen Doggett, Author provided

Australian climate is characterised by its variability, however we have experienced a general trend towards increased spring and summer monsoonal rain across northern Australia, and decreased late autumn and winter rainfall in the south.

Flooding rains

Kunjin virus is mainly transmitted by a small mosquito called Culex annulirostris, the common banded mosquito, in Australia. We are lucky because human infection rarely causes disease, even though Kunjin and the common-banded mosquito are widespread in Australia.

Kunjin’s close relative, the US strain of West Nile Virus is much more virulent, causing more human disease. These viruses are well known for their ability to mutate quickly, so they are always keeping medical authorities on their toes.

Higher than average rainfall and flooding in eastern Australia in the second half of 2010 and 2011 provided ideal conditions for breeding common banded mosquitoes, and in 2011 a dangerous strain of Kunjin appeared that caused acute encephalitis (swelling of the brain) in horses. This disease has only been detected in one human, however this mosquito feeds on both humans and horses.

This new virulent strain of Kunjin also appeared in new areas east of the Great Dividing Range, suggesting other unknown changes in transmission.

As temperatures increase, mosquito activity will begin earlier in the season and reach higher levels of abundance sooner, and maintain higher populations longer. These factors will all probably tend to increase the rate of transmission of Kunjin to both humans and animals.

Drying out

While flooding may have helped spread Kunjin, drought may have helped another mosquito-borne virus.

It would be simple to assume that drought would reduce mosquito populations by reducing the larval habitat (water), and thereby reduce the incidence of mosquito-borne disease in Australia.

However, this is not necessarily the case. Another Australian mosquito, Aedes notoscriptus, the striped mosquito, is responsible for transmitting Ross River and Barmah Forest Virus in Australia.

Mosquitoes will breed wherever there is standing water.
Suzanne Phillips/Flickr, CC BY-NC

The striped mosquito is unusual in comparison to its cousins because it breeds in small containers of water, such as tree holes in natural environments. The main carrier of Dengue in Australia, Aedes aegypti, shares this habit.

These small container habitats abound in Australia’s urban backyard, with water features, water and food bowls for pets, and various toys providing such breeding places.

With the drought, Australians became much more water wise, and installed various water storage devices in their gardens, ranging from buckets left out in a storm, to professionally installed rain tanks. All these are potential habitat for the striped mosquito to breed.

In this case drought has caused an increase in the abundance of a mosquito virus carrier because of a change in human behaviour.

The return of Dengue?

Dengue fever is transmitted in Australia by the mosquito Aedes aegypti. The mosquito is restricted to Queensland, and Dengue fever transmission is restricted to coastal northern Queensland.

Recent modelling predicts that moderate climate change would extend the Dengue risk zone to Brisbane, exposing much larger human populations to risk.

However, before the 1930s, Dengue fever transmission was known south almost to Sydney, and Aedes aegypti was known throughout mainland Australia except the deserts.

Both the mosquito, and the disease, have retreated to Queensland since then, and we don’t know why. What is clear is that we don’t really understand what controls the distribution of Aedes aegypti or Dengue in Australia, but given the contraction of the disease in historical time, it is unlikely that a warming climate will produce a simple response in the insect or the disease.

Australian insects will be affected by climate change, but simple predictions based on increasing average temperatures and changing rainfall patterns miss the important effects of complex biological interactions.

In addition, we are only just beginning to use models that are sophisticated enough to consider how insects might evolve under changing climate.

Investing in a deeper understanding of these complex biological webs, and their outcomes for human society, will result in great returns. Our predictions of the future state of Australian plants and animals will become more accurate and we will also improve human health and manage our biodiversity more sustainably into the future.

The Conversation

David Yeates is Director of the Australian National Insect Collection at CSIRO.

This article was originally published on The Conversation.
Read the original article.

We are learning mosquitoes are basically invincible


Mosquitoes are, at best, horrible annoyances. At worst? They are genocidal maniacs, responsible for more than half a million deaths a year, transmitting malaria and other diseases. Were causing extinction subject to popular vote, mosquitoes would win in a landslide.

All of that, relative to the moment, is the good news. Now, the bad.

Mosquitoes laugh at your so-called repellant.

Well, they don’t laugh, as such, lacking the capacity for forced expulsion of air from their probosci and, likewise, any sense of humor. Point is, the most common chemical used to repel the little idiots is losing its effectiveness. From

A group of researchers from the London School of Hygiene and Tropical Medicine discovered that three hours after an exposure to DEET, many Aedes aegypti mosquitoes were immune to the chemical, ignoring its typically noxious smell and attempting to land on irresistible human skin. …

So why…

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